Collect 100 mL of water

Fill the jar almost to the top with the water you're testing. It holds about 100 mL (3.5 oz). Leave room for the 1 mL vial you add next. Results are reported per 100 mL, so use the full jar.
Instructions
One sample at a time. Most of the 30 minutes is waiting after you add the vial.
Two parts. Tap one to open it.

Fill the jar almost to the top with the water you're testing. It holds about 100 mL (3.5 oz). Leave room for the 1 mL vial you add next. Results are reported per 100 mL, so use the full jar.

Add the vial, swirl gently, and wait 15–20 minutes. Longer is better; the dye needs time to bind.
Cover the jar while you wait. Dust from the air lands in an open jar and gets counted.

Unscrew the holder, set the round filter membrane inside, and screw it back together.
Choose the syringe connector that matches yours, then watch the short video before attaching the membrane filter holder.
Which connector does your syringe have?

Pour the prepared water into the top of the syringe.
If you have a 60 mL syringe, fill and push it roughly twice. If you have a 100 mL syringe, fill and push it once. Either way, keep going until the jar is empty so the full 100 mL sample passes through the same filter.
Unscrew the filter holder before you pull the plunger back for the second fill. Pulling against the filter can damage it.

Push the water through right away; don't let it sit.

Unscrew the holder and take out the filter. Keep the side that faced the water pointing up. That's the side you read.
These details can make particles harder to see. If the screen looks unusual, check both before reading the result.
If the 25 mm filter disc curls or buckles, the light can hit it unevenly and create darker areas. Handle it with the tweezers and try to keep it very flat while you read it.
Dab it with a paper towel until slightly damp. Damp makes the disc stick flat; bone-dry, it curls.
The flashlight is fixed in place, so aiming isn't the problem — the batteries are. Below about 50–70% charge the blue light stops exciting the dye and nothing glows, even with plastic on the filter. You won't see it happen: a half-dead cell still throws a beam that looks bright. If the kit was finding particles and then stopped, put in fresh batteries before you trust a clean result.
This video uses the previous version of the kit, but the water-testing process is the same: add the dye, check the blank filter, push the water through, and count what glows. Watch the blank check at 0:40. Look at the filter before any water runs through it, count anything already glowing, and subtract that from your final number.
Have two tests? Run one on unfiltered tap water and one after your filter.
Wash the jar thoroughly. Don't drink from it.
Suspected microplastics can appear as small glowing dots, fibers, or dark marks. Use these views as a reference while reading the filter.
The color varies with the lens and the light: deep red, warm orange, or almost blue. Every view below counts.
What you're looking at
A long fiber may be polyester, or it may be cotton or rayon, which aren't plastics, or a clothing fiber that landed from the air while your sample sat open.
Carbon shed by a carbon filter, grit, rust and other debris all look like black specks at 10x, the same as black plastic. Looking at one doesn't tell you which it is. More on this.














These images do not show any microplastics. An even view with no distinct glowing dots — nothing to count.





Each refill covers one more run — keep the microscope, syringe, and filter holder you already have. sold separately.
Stuck on a step? Use the chat in the bottom-right corner.
Don't have a kit yet? The microplastics test kit is available to order online. Tests are sold separately.
Using the previous digital-microscope rig? Open the legacy instructions.